Human CD141(+) (BDCA-3)(+) dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens

Show simple item record Jongbloed, SL en Kassianos, AJ en McDonald, KJ en Clark, GJ en Ju, XS en Angel, Catherine en Chen, Chun-Jen en Dunbar, Peter en Wadley, RB en Jeet, V en Vulink, AJE en Hart, DNJ en Radford, KJ en 2011-11-30T00:27:29Z en 2010-06-07 en
dc.identifier.citation Journal of Experimental Medicine 207(6):1247-1260 07 Jun 2010 en
dc.identifier.issn 0022-1007 en
dc.identifier.uri en
dc.description.abstract The characterization of human dendritic cell (DC) subsets is essential for the design of new vaccines. We report the first detailed functional analysis of the human CD141+ DC subset. CD141+ DCs are found in human lymph nodes, bone marrow, tonsil, and blood, and the latter proved to be the best source of highly purified cells for functional analysis. They are characterized by high expression of toll-like receptor 3, production of IL-12p70 and IFN-β, and superior capacity to induce T helper 1 cell responses, when compared with the more commonly studied CD1c+ DC subset. Polyinosine-polycytidylic acid (poly I:C)–activated CD141+ DCs have a superior capacity to cross-present soluble protein antigen (Ag) to CD8+ cytotoxic T lymphocytes than poly I:C–activated CD1c+ DCs. Importantly, CD141+ DCs, but not CD1c+ DCs, were endowed with the capacity to cross-present viral Ag after their uptake of necrotic virus-infected cells. These findings establish the CD141+ DC subset as an important functionally distinct human DC subtype with characteristics similar to those of the mouse CD8α+ DC subset. The data demonstrate a role for CD141+ DCs in the induction of cytotoxic T lymphocyte responses and suggest that they may be the most relevant targets for vaccination against cancers, viruses, and other pathogens. en
dc.language EN en
dc.publisher Rockefeller University Press en
dc.relation.ispartofseries Journal of Experimental Medicine en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.subject TOLL-LIKE RECEPTOR-3 en
dc.subject C-TYPE LECTIN en
dc.subject IN-VIVO en
dc.subject T-CELLS en
dc.subject IMMUNE-RESPONSE en
dc.subject IFN-GAMMA en
dc.subject CD8(+) en
dc.subject BLOOD en
dc.subject CD8-ALPHA(+) en
dc.title Human CD141(+) (BDCA-3)(+) dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens en
dc.type Journal Article en
dc.identifier.doi 10.1084/jem.20092140 en
pubs.issue 6 en
pubs.begin-page 1247 en
pubs.volume 207 en
dc.rights.holder Copyright: The Rockefeller University Press; The Authors en
dc.identifier.pmid 20479116 en en
pubs.end-page 1260 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 119569 en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1540-9538 en
pubs.record-created-at-source-date 2011-11-30 en
pubs.dimensions-id 20479116 en

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