dc.contributor.author |
Ahn, S |
en |
dc.contributor.author |
Moniot, S |
en |
dc.contributor.author |
Elias, M |
en |
dc.contributor.author |
Chabriere, E |
en |
dc.contributor.author |
Kim, D |
en |
dc.contributor.author |
Scott, George |
en |
dc.date.accessioned |
2011-11-30T00:29:50Z |
en |
dc.date.issued |
2007-07-24 |
en |
dc.identifier.citation |
FEBS Letters 581(18):3455-3460 24 Jul 2007 |
en |
dc.identifier.issn |
0014-5793 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/9648 |
en |
dc.description.abstract |
A recombinant DING protein from Pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. Here we report the three-dimensional structure of the protein, confirming a close similarity to the "Venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. Site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. Deletion of one of the two hinged domains that constitute the Venus flytrap also eliminates phosphate binding whilst enhancing mitogenic activity. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
en |
dc.language |
EN |
en |
dc.publisher |
Elsevier Science B.V. |
en |
dc.relation.ispartofseries |
FEBS Letters |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from
http://www.sherpa.ac.uk/romeo/issn/0014-5793/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
DING protein |
en |
dc.subject |
phosphate binding |
en |
dc.subject |
human phosphate-binding protein |
en |
dc.subject |
pstS protein |
en |
dc.subject |
SITE-DIRECTED MUTAGENESIS |
en |
dc.subject |
PHOSPHATE-BINDING PROTEIN |
en |
dc.subject |
PARAOXONASE PON1 |
en |
dc.subject |
TRANSPORT |
en |
dc.subject |
SPECIFICITY |
en |
dc.subject |
RECEPTORS |
en |
dc.title |
Structure-function relationships in a bacterial DING protein |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.febslet.2007.06.050 |
en |
pubs.issue |
18 |
en |
pubs.begin-page |
3455 |
en |
pubs.volume |
581 |
en |
dc.rights.holder |
Copyright: 2007 Federation of European Biochemical Societies; Elsevier B.V |
en |
dc.identifier.pmid |
17612529 |
en |
pubs.end-page |
3460 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
76494 |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
17612529 |
en |