Structure-function relationships in a bacterial DING protein

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dc.contributor.author Ahn, S en
dc.contributor.author Moniot, S en
dc.contributor.author Elias, M en
dc.contributor.author Chabriere, E en
dc.contributor.author Kim, D en
dc.contributor.author Scott, George en
dc.date.accessioned 2011-11-30T00:29:50Z en
dc.date.issued 2007-07-24 en
dc.identifier.citation FEBS Letters 581(18):3455-3460 24 Jul 2007 en
dc.identifier.issn 0014-5793 en
dc.identifier.uri http://hdl.handle.net/2292/9648 en
dc.description.abstract A recombinant DING protein from Pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. Here we report the three-dimensional structure of the protein, confirming a close similarity to the "Venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. Site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. Deletion of one of the two hinged domains that constitute the Venus flytrap also eliminates phosphate binding whilst enhancing mitogenic activity. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. en
dc.language EN en
dc.publisher Elsevier Science B.V. en
dc.relation.ispartofseries FEBS Letters en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0014-5793/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject DING protein en
dc.subject phosphate binding en
dc.subject human phosphate-binding protein en
dc.subject pstS protein en
dc.subject SITE-DIRECTED MUTAGENESIS en
dc.subject PHOSPHATE-BINDING PROTEIN en
dc.subject PARAOXONASE PON1 en
dc.subject TRANSPORT en
dc.subject SPECIFICITY en
dc.subject RECEPTORS en
dc.title Structure-function relationships in a bacterial DING protein en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.febslet.2007.06.050 en
pubs.issue 18 en
pubs.begin-page 3455 en
pubs.volume 581 en
dc.rights.holder Copyright: 2007 Federation of European Biochemical Societies; Elsevier B.V en
dc.identifier.pmid 17612529 en
pubs.end-page 3460 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 76494 en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 17612529 en


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