dc.contributor.author |
Wlodkowic, Donald |
en |
dc.contributor.author |
Skommer, Joanna |
en |
dc.contributor.author |
Darzynkiewicz, Z |
en |
dc.coverage.spatial |
United States |
en |
dc.date.accessioned |
2011-12-01T23:18:46Z |
en |
dc.date.issued |
2011 |
en |
dc.identifier.citation |
Methods in Molecular Biology 740:81-89 201 |
en |
dc.identifier.issn |
1064-3745 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/9686 |
en |
dc.description.abstract |
The gross majority of classical apoptotic hallmarks can be rapidly examined by multiparameter flow cytometry. As a result, cytometry became a technology of choice in diverse studies of cellular demise. In this context, a novel class of substituted unsymmetrical cyanine SYTO probes has recently become commercially available. Derived from thiazole orange, SYTO display low intrinsic fluorescence, with strong enhancement upon binding to DNA and/or RNA. Broad selection of excitation/emission spectra has recently driven implementation of SYTO dyes in polychromatic protocols with the detection of apoptosis being one of the most prominent applications In this chapter, we outline a handful of commonly used protocols for the assessment of apoptotic events using selected SYTO probes (SYTO 16, 62, 80) in conjunction with common plasma membrane permeability markers (PI, YO-PRO 1, 7-AAD). |
en |
dc.language |
eng |
en |
dc.publisher |
humana press (springer imprint) |
en |
dc.relation.ispartofseries |
Methods in Molecular Biology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1064-3745/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Apoptosis |
en |
dc.subject |
Benzoxazoles |
en |
dc.subject |
Cell Survival |
en |
dc.subject |
Cytological Techniques |
en |
dc.subject |
Dactinomycin |
en |
dc.subject |
Fluorescent Dyes |
en |
dc.subject |
Humans |
en |
dc.subject |
Lymphoma, B-Cell |
en |
dc.subject |
Molecular Probes |
en |
dc.subject |
Propidium |
en |
dc.subject |
Quinolinium Compounds |
en |
dc.subject |
Tumor Cells, Cultured |
en |
dc.title |
Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/978-1-61779-108-6_10 |
en |
pubs.begin-page |
81 |
en |
pubs.volume |
740 |
en |
dc.rights.holder |
Copyright: Springer Science+Business Media, LLC |
en |
dc.identifier.pmid |
21468970 |
en |
pubs.end-page |
89 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
230065 |
en |
dc.identifier.eissn |
1940-6029 |
en |
pubs.record-created-at-source-date |
2011-12-02 |
en |
pubs.dimensions-id |
21468970 |
en |