Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry.

Show simple item record

dc.contributor.author Wlodkowic, Donald en
dc.contributor.author Skommer, Joanna en
dc.contributor.author Faley, S en
dc.contributor.author Darzynkiewicz, Z en
dc.contributor.author Cooper, JM en
dc.coverage.spatial United States en
dc.date.accessioned 2011-12-01T23:20:28Z en
dc.date.issued 2009-06-10 en
dc.identifier.citation Experimental Cell Research 315(10):1706-1714 10 Jun 2009 en
dc.identifier.issn 0014-4827 en
dc.identifier.uri http://hdl.handle.net/2292/9692 en
dc.description.abstract Cell death is a stochastic process, often initiated and/or executed in a multi-pathway/multi-organelle fashion. Therefore, high-throughput single-cell analysis platforms are required to provide detailed characterization of kinetics and mechanisms of cell death in heterogeneous cell populations. However, there is still a largely unmet need for inert fluorescent probes, suitable for prolonged kinetic studies. Here, we compare the use of innovative adaptation of unsymmetrical SYTO dyes for dynamic real-time analysis of apoptosis in conventional as well as microfluidic chip-based systems. We show that cyanine SYTO probes allow non-invasive tracking of intracellular events over extended time. Easy handling and "stain-no wash" protocols open up new opportunities for high-throughput analysis and live-cell sorting. Furthermore, SYTO probes are easily adaptable for detection of cell death using automated microfluidic chip-based cytometry. Overall, the combined use of SYTO probes and state-of-the-art Lab-on-a-Chip platform emerges as a cost effective solution for automated drug screening compared to conventional Annexin V or TUNEL assays. In particular, it should allow for dynamic analysis of samples where low cell number has so far been an obstacle, e.g. primary cancer stems cells or circulating minimal residual tumors. en
dc.language eng en
dc.publisher Elsevier Inc. en
dc.relation.ispartofseries Experimental Cell Research en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0014-4827/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Apoptosis en
dc.subject Biological Assay en
dc.subject Carbocyanines en
dc.subject Cell Death en
dc.subject Cell Line, Tumor en
dc.subject Cell Survival en
dc.subject Flow Cytometry en
dc.subject Fluorescent Dyes en
dc.subject Humans en
dc.subject Intracellular Space en
dc.subject Microchip Analytical Procedures en
dc.subject Microfluidics en
dc.subject Time Factors en
dc.title Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.yexcr.2009.03.006 en
pubs.issue 10 en
pubs.begin-page 1706 en
pubs.volume 315 en
dc.rights.holder Copyright: 2009 Elsevier Inc. en
dc.identifier.pmid 19298813 en
pubs.end-page 1714 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 230050 en
dc.identifier.eissn 1090-2422 en
dc.identifier.pii S0014-4827(09)00112-8 en
pubs.record-created-at-source-date 2011-12-02 en
pubs.dimensions-id 19298813 en

Files in this item

There are no files associated with this item.

Find Full text

This item appears in the following Collection(s)

Show simple item record


Search ResearchSpace