dc.contributor.author |
Wlodkowic, Donald |
en |
dc.contributor.author |
Skommer, Joanna |
en |
dc.contributor.author |
Faley, S |
en |
dc.contributor.author |
Darzynkiewicz, Z |
en |
dc.contributor.author |
Cooper, JM |
en |
dc.coverage.spatial |
United States |
en |
dc.date.accessioned |
2011-12-01T23:20:28Z |
en |
dc.date.issued |
2009-06-10 |
en |
dc.identifier.citation |
Experimental Cell Research 315(10):1706-1714 10 Jun 2009 |
en |
dc.identifier.issn |
0014-4827 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/9692 |
en |
dc.description.abstract |
Cell death is a stochastic process, often initiated and/or executed in a multi-pathway/multi-organelle fashion. Therefore, high-throughput single-cell analysis platforms are required to provide detailed characterization of kinetics and mechanisms of cell death in heterogeneous cell populations. However, there is still a largely unmet need for inert fluorescent probes, suitable for prolonged kinetic studies. Here, we compare the use of innovative adaptation of unsymmetrical SYTO dyes for dynamic real-time analysis of apoptosis in conventional as well as microfluidic chip-based systems. We show that cyanine SYTO probes allow non-invasive tracking of intracellular events over extended time. Easy handling and "stain-no wash" protocols open up new opportunities for high-throughput analysis and live-cell sorting. Furthermore, SYTO probes are easily adaptable for detection of cell death using automated microfluidic chip-based cytometry. Overall, the combined use of SYTO probes and state-of-the-art Lab-on-a-Chip platform emerges as a cost effective solution for automated drug screening compared to conventional Annexin V or TUNEL assays. In particular, it should allow for dynamic analysis of samples where low cell number has so far been an obstacle, e.g. primary cancer stems cells or circulating minimal residual tumors. |
en |
dc.language |
eng |
en |
dc.publisher |
Elsevier Inc. |
en |
dc.relation.ispartofseries |
Experimental Cell Research |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0014-4827/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Apoptosis |
en |
dc.subject |
Biological Assay |
en |
dc.subject |
Carbocyanines |
en |
dc.subject |
Cell Death |
en |
dc.subject |
Cell Line, Tumor |
en |
dc.subject |
Cell Survival |
en |
dc.subject |
Flow Cytometry |
en |
dc.subject |
Fluorescent Dyes |
en |
dc.subject |
Humans |
en |
dc.subject |
Intracellular Space |
en |
dc.subject |
Microchip Analytical Procedures |
en |
dc.subject |
Microfluidics |
en |
dc.subject |
Time Factors |
en |
dc.title |
Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.yexcr.2009.03.006 |
en |
pubs.issue |
10 |
en |
pubs.begin-page |
1706 |
en |
pubs.volume |
315 |
en |
dc.rights.holder |
Copyright: 2009 Elsevier Inc. |
en |
dc.identifier.pmid |
19298813 |
en |
pubs.end-page |
1714 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
230050 |
en |
dc.identifier.eissn |
1090-2422 |
en |
dc.identifier.pii |
S0014-4827(09)00112-8 |
en |
pubs.record-created-at-source-date |
2011-12-02 |
en |
pubs.dimensions-id |
19298813 |
en |