Flow cytometry-based apoptosis detection.

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dc.contributor.author Wlodkowic, Donald en
dc.contributor.author Skommer, Joanna en
dc.contributor.author Darzynkiewicz, Z en
dc.coverage.spatial United States en
dc.date.accessioned 2011-12-01T23:21:01Z en
dc.date.issued 2009 en
dc.identifier.citation Methods in Molecular Biology 559(1):19-32 2009 en
dc.identifier.issn 1064-3745 en
dc.identifier.uri http://hdl.handle.net/2292/9693 en
dc.description.abstract An apoptosing cell demonstrates multitude of characteristic morphological and biochemical features, which vary depending on the stimuli and the cell type. The gross majority of classical apoptotic hallmarks can be rapidly examined by flow and image cytometry. Cytometry thus became a technology of choice in diverse studies of cellular demise. A large variety of cytometric methods designed to identify apoptotic cells and probe mechanisms associated with this mode of cell demise have been developed during the past two decades. In the present chapter, we outline a handful of commonly used methods that are based on the assessment of: mitochondrial transmembrane potential, activation of caspases, plasma membrane alterations and DNA fragmentation. en
dc.language eng en
dc.publisher humana press (springer imprint) en
dc.relation.ispartofseries Methods in Molecular Biology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1064-3745/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Animals en
dc.subject Annexin A5 en
dc.subject Apoptosis en
dc.subject Caspases en
dc.subject Cell Membrane en
dc.subject DNA Fragmentation en
dc.subject Flow Cytometry en
dc.subject Humans en
dc.subject Membrane Potential, Mitochondrial en
dc.title Flow cytometry-based apoptosis detection. en
dc.type Journal Article en
dc.identifier.doi 10.1007/978-1-60327-017-5_2 en
pubs.issue 1 en
pubs.begin-page 19 en
pubs.volume 559 en
dc.rights.holder Copyright: Humana Press, a part of Springer Science +Business Media, LLC en
dc.identifier.pmid 19609746 en
pubs.end-page 32 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 230055 en
pubs.record-created-at-source-date 2011-12-02 en
pubs.dimensions-id 19609746 en

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