Microfabricated analytical systems for integrated cancer cytomics.

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dc.contributor.author Wlodkowic, Donald en
dc.contributor.author Cooper, JM en
dc.coverage.spatial Germany en
dc.date.accessioned 2011-12-01T23:34:28Z en
dc.date.issued 2010-09 en
dc.identifier.citation Analytical and Bioanalytical Chemistry 398(1):193-209 Sep 2010 en
dc.identifier.issn 1618-2642 en
dc.identifier.uri http://hdl.handle.net/2292/9708 en
dc.description.abstract Tracking and understanding cell-to-cell variability is fundamental for systems biology, cytomics and computational modelling that aids e.g. anti-cancer drug discovery. Limitations of conventional cell-based techniques, such as flow cytometry and single cell imaging, however, make the high-throughput dynamic analysis on cellular and subcellular processes tedious and exceedingly expensive. The development of microfluidic lab-on-a-chip technologies is one of the most innovative and cost-effective approaches towards integrated cytomics. Lab-on-a-chip devices promise greatly reduced costs, increased sensitivity and ultrahigh throughput by implementing parallel sample processing. The application of laminar fluid flow under low Reynolds numbers provides an attractive analytical avenue for the rapid delivery and exchange of reagents with exceptional accuracy. Under these conditions, the fluid flow has no inertia, enabling the precise dosing of drugs, both spatially and temporally. In addition, by confining the dimensions of the microfluidic structure, it is possible to facilitate the precise sequential delivery of drugs and/or functional probes into the cellular systems. As only low cell numbers and operational reagent volumes are required, high-throughput integrated cytomics on a single cell level finally appears within the reach of clinical diagnostics and drug screening routines. Lab-on-a-chip microfluidic technologies therefore provide new opportunities for the development of content-rich personalized clinical diagnostics and cost-effective drug discovery. It is largely anticipated that advances in microfluidic technologies should aid in tailoring of investigational therapies and support the current computational efforts in systems biology. en
dc.language eng en
dc.publisher Springer Verlag (Germany) en
dc.relation.ispartofseries Analytical and Bioanalytical Chemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1618-2642/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Animals en
dc.subject Humans en
dc.subject Lab-On-A-Chip Devices en
dc.subject Microarray Analysis en
dc.subject Microfluidic Analytical Techniques en
dc.subject Microtechnology en
dc.subject Neoplasms en
dc.title Microfabricated analytical systems for integrated cancer cytomics. en
dc.type Journal Article en
dc.identifier.doi 10.1007/s00216-010-3722-8 en
pubs.issue 1 en
pubs.begin-page 193 en
pubs.volume 398 en
dc.rights.holder Copyright: Springer-Verlag en
dc.identifier.pmid 20419489 en
pubs.end-page 209 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Review en
pubs.elements-id 230062 en
dc.identifier.eissn 1618-2650 en
pubs.record-created-at-source-date 2011-12-02 en
pubs.dimensions-id 20419489 en

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