Metabolomics-on-a-chip: integrating microfluidic single cell culture with mass spectroscopy

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dc.contributor.author Wlodkowic, Donald en
dc.contributor.author Wright, BEW en
dc.contributor.author Akagi, JA en
dc.contributor.author Greenwood, DG en
dc.contributor.author Villas-Boas, SVB en
dc.contributor.author Williams, DEW en
dc.coverage.spatial Melbourne, Australia en
dc.date.accessioned 2011-12-01T23:35:24Z en
dc.date.issued 2010 en
dc.identifier.citation 2nd Australasian Symposium on Metabolomics, Melbourne, Australia. 2010 en
dc.identifier.uri http://hdl.handle.net/2292/9712 en
dc.description.abstract We are developing microfluidic systems to study the metabolome of a single cell or small cell groups in well-defined culture conditions. [1] Microfluidic systems, also called labs-on-a-chip, are constructed on the same scale as cells (features in the range of 1-100 µm) cell culture numbers with high spatial and temporal control of culture conditions. [2] Toward this end, we have adapted the use of microfluidic single cell traps capable of capturing and housing the culture of a single cell or small cell groups using polydimethysiloxane (PDMS) based microfluidics. [3] Further, we have integrated microfluidic cell culture with novel monolithic PDMS electrospray ionisation (ESI) emitters. We have tested the fully integrated cell traps/ESI emitters using both test solutions and in trial runs on small yeast cell groups using an ion trap and fourier transform ion cyclotron resonance mass spectrometer. Initial results on the microfluidic cell traps, microfluidic-ESI characteristics, and performance of the integrated cell traps/ESI system will be presented along with an comparison of conventional nanospray-ESI with the integrate microfluidic system with emphasis placed on detection sensitivity and interference effects from PDMS. en
dc.relation.ispartof 2nd Australasian Symposium on Metabolomics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Metabolomics-on-a-chip: integrating microfluidic single cell culture with mass spectroscopy en
dc.type Conference Item en
dc.rights.holder Copyright: the author en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Abstract en
pubs.elements-id 249865 en
pubs.record-created-at-source-date 2011-12-02 en


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