Real-Time Fluorescent Imaging of Apoptosis using Chip-Based Technologies

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dc.contributor.author Wlodkowic, Donald en
dc.contributor.author Zhao, HZ en
dc.contributor.author Akagi, JA en
dc.contributor.author Dobrucki, JD en
dc.contributor.author Williams, DEW en
dc.contributor.author Cooper, JMC en
dc.contributor.author Darzynkiewicz, ZD en
dc.coverage.spatial Sydney, Australia en
dc.date.accessioned 2011-12-01T23:38:43Z en
dc.date.issued 2011 en
dc.identifier.citation AMNFC - 2nd Australian & New Zealand Micro and Nanofluidics Symposium, Sydney, Australia. 2011 en
dc.identifier.uri http://hdl.handle.net/2292/9720 en
dc.description.abstract Validation of potential therapeutic targets necessitates the development of new assays that provide both spatial and temporal relationships in signalling networks. Cell-based assays are therefore becoming an important part of the postgenomic biomedical research. The present study was designed to provide a mechanistic rationale for the kinetic assays and to assess their potential to obtain dynamic and single-cell data on the stochastic process of cell death on Lab-on-a-Chip (LOC) technologies. We propose an innovative approach to dynamically trace cell death in real-time using fluorochromes such as propidium iodide (PI), SYTOX Green, SYTOX Red, YO-PRO 1, Annexin V and TMRM. We also demonstrate that, when used with innovative bioassays, microfluidic dynamic live-cell analysis is a practical alternative for multiparameter studies on a single-cell level. The LOC devices not only reduce the complexity of conventional cell culture protocols but also enabled time-resolved studies on apoptosis. The adaptation of non-toxic biomarkers that can continuously circulate inside enclosed microculture system will be beneficial for the advancement of up and coming LOC technologies. Our data supports the hypothesis that real-time bioassays in combination with LOC devices allow for rapid and simple analysis of cell death, particularly useful if the death pattern is a stochastic rather than deterministic process. As a result, they provide sensitivity that often cannot be achieved with conventional end-point analysis. en
dc.relation.ispartof AMNFC - 2nd Australian & New Zealand Micro and Nanofluidics Symposium en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Real-Time Fluorescent Imaging of Apoptosis using Chip-Based Technologies en
dc.type Conference Item en
dc.description.version author's version en
dc.rights.holder Copyright: the author en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Abstract en
pubs.elements-id 249893 en
pubs.record-created-at-source-date 2011-12-02 en


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