dc.contributor.author |
Wlodkowic, Donald |
en |
dc.contributor.author |
Khoshmanesh, KK |
en |
dc.contributor.author |
Akagi, JA |
en |
dc.contributor.author |
Nahavandi, SN |
en |
dc.contributor.author |
Kalantar-Zadeh, KKZ |
en |
dc.contributor.author |
Williams, DEW |
en |
dc.contributor.author |
Cooper, JMC |
en |
dc.coverage.spatial |
Sydney, Australia |
en |
dc.date.accessioned |
2011-12-01T23:38:59Z |
en |
dc.date.issued |
2011 |
en |
dc.identifier.citation |
AMNFC - 2nd Australian & New Zealand Micro and Nanofluidics Symposium, Sydney, Australia. 2011 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/9721 |
en |
dc.description.abstract |
Live cell assays are an important part of drug discovery pipelines and personalized point-of-care diagnostics. In this context, Lab-on-a-Chip (LOC) devices are being widely considered as emerging technologies that can support massively parallel analysis at a single cell level. Reportedly they provide unique capabilities to monitor single-cell signalling dynamics, especially in rare subpopulations such as cancer cells or haematopoietic stem cells. Despite a large body of evidence on the fluorescence imaging, impendence and optical spectroscopies for characterisation of single living cells on a chip, however, no attempts have been so far made to interface microfabricated chip-based technologies with environmental scanning electron microscopy (ESEM). Here we for the first time describe the development of a non-invasive and simplified DEP chip that can be interfaced with ESEM imaging to provide analysis of non-adherent cells immobilised in the positive DEP (pDEP) fields. DEP microelectrode arrays were fabricated using thin films of chrome/gold deposited to a thickness of 500 Å/1500 Å on the glass substrate using electron beam evaporation process. Chip-based arrays were applied for ESEM analysis of DEP-immobilised human leukaemic cells. Most importantly non-adherent cells remain viable during processing and are well retained during imaging. Our innovative approach avoids extensive preparative procedures, is easy to perform for non-specialised personnel and thus prospectively amenable for automation. |
en |
dc.relation.ispartof |
AMNFC - 2nd Australian & New Zealand Micro and Nanofluidics Symposium |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Interfacing dielectrophoretic cell trapping arrays with ESEM |
en |
dc.type |
Presentation |
en |
dc.rights.holder |
Copyright: the author |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Conference Oral Presentation |
en |
pubs.elements-id |
249891 |
en |
pubs.record-created-at-source-date |
2011-12-02 |
en |