Dynamic Analysis of Tumour Cell Death Using Dielectrophoresis

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dc.contributor.author Wlodkowic, Donald en
dc.contributor.author Khoshmanesh, KK en
dc.contributor.author Akagi, JA en
dc.contributor.author Nahavandi, SN en
dc.contributor.author Skommer, JS en
dc.contributor.author Baratchi, SB en
dc.contributor.author Cooper, JMC en
dc.contributor.author Kalantar-Zadeh, KKZ en
dc.contributor.author Williams, DEW en
dc.coverage.spatial Sydney, Australia en
dc.date.accessioned 2011-12-01T23:39:40Z en
dc.date.issued 2011 en
dc.identifier.citation AMNFC - 2nd Australian & New Zealand Micro and Nanofluidics Symposium, Sydney, Australia. 2011 en
dc.identifier.uri http://hdl.handle.net/2292/9724 en
dc.description.abstract The important discovery that induction of programmed cell death in neoplastic cells is a critical event that defines tumour growth rate and response to anti-cancer therapy has provided a framework for the rationally designed, molecular anti-cancer therapeutics. The regulation of cancer cell death involves the circuitry of feedback events and rapid switches between analogue and digital responses. Such enormous complexity, with numerous variables acting at the same time, requires multiparametric and dynamic analysis at a single cell level. The opportunity to dynamically quantify individual cellular states is advantageous over the commonly employed static, end-point assays. We describe here the development and application of a microfabricated, dielectrophoretic (DEP) cell immobilisation platform for the real-time analysis of tumour cell death. We applied this technology to the dynamic analysis of haematopoietic tumour cells that represent a particular challenge for real-time imaging due to their dislodgement during image acquisition. The present study was designed to provide a comprehensive mechanistic rationale for accelerated cell-based assays on DEP chips using real-time labelling with cell permeability markers. Results indicate that simple DEP cell immobilisation technology can be readily applied for the dynamic analysis of investigational drugs in hematopoietic cancer cells. en
dc.relation.ispartof AMNFC - 2nd Australian & New Zealand Micro and Nanofluidics Symposium en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Dynamic Analysis of Tumour Cell Death Using Dielectrophoresis en
dc.type Presentation en
dc.rights.holder Copyright: the author en
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype Conference Oral Presentation en
pubs.elements-id 249885 en
pubs.record-created-at-source-date 2011-12-02 en

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