Gray, D.HSkunner, S.J.M.Katz, June Margaret2008-10-292008-10-291983Thesis (PhD--Surgery)--University of Auckland, 1983http://hdl.handle.net/2292/3125Restricted Item. Print thesis available in the University of Auckland Library or may be available through Interlibrary Loan.Calvaria dissected from 6-day-old mice were maintained in organ culture in a chemically-defined medium without serum additives. Bone resorption was quantified by the release of 45Ca or hydroxyproline (Hyp) into the medium. Collagen synthesis (Hyp) in bone was estimated by the incorporation of [5-3H]-proline. Prostaglandin E2 (PGE2), prostaglandin F (PGF) and 6-keto-prostaglandin F1a (6-keto-PGF1a) released into the medium from cultured calvaria, were measured by radioimmunoassay. Prostanoids were also quantified by the release of radioactively-labelled metabolites from calvaria pre-labelled in vitro with [3H]-arachidonic acid. These metabolites were separated by high performance liquid chromatography.enRestricted Item. Print thesis available in the University of Auckland Library or may be available through Interlibrary Loan.https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htmThesisFields of Research::320000 Medical and Health Sciences::321000 Clinical Sciences::321029 SurgeryCopyright: The authorhttp://purl.org/eprint/accessRights/ClosedAccessQ112847390