Wang, SenyeZhou, LiAttia, Fatma Al-Zahra KKTang, QiWang, MengkeLiu, ZhenhuaWaterhouse, Geoffrey INLiu, LijunKang, Wenyi2022-12-162022-12-162021-01(2021). Frontiers in Nutrition, 8, 748031-.2296-861Xhttps://hdl.handle.net/2292/62165<i>Origanum majorana</i> L. is an aromatic herb that has been grown in several Mediterranean countries since ancient times, but became popular during the Middle Ages as a medicinal plant and seasoning ingredient. <i>O. majorana</i> has many pharmacological effects, but its immunoreactive components and mechanisms are still unclear. In this study, four compounds were isolated and identified from <i>O. majorana</i> by a spectral analysis, including ¹H and ¹³C-NMR. They were 1H-indole-2-carboxylic acid (<b>1</b>), (+)-laricresol (<b>2</b>), (+)-isolaricresol (<b>3</b>), and procumboside B (<b>4</b>, pB), which were isolated for the first time in <i>O. majorana</i>. The immunomodulatory effects of the four compounds were screened, and pB had good immunomodulatory activity on RAW 264.7 cells. The immunomodulatory mechanism of pB was proved, in which pB could increase the secretion of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and reactive oxygen species (ROS) and simultaneously upregulate the expression of CD80 and CD86 on the cell surface. These results suggested that the mechanism of pB may be related to the activation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPKs)-signaling pathways. <i>O. majorana</i> is rich in nutrients and is commonly used in diets, so it can be used as a nutritional supplement with immunomodulatory effects.Electronic-eCollectionItems in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htmhttps://creativecommons.org/licenses/by/4.0/MAPKsNF-κBOriganum majorana L.RAW264.7immuneprocumboside BComplementary and Integrative HealthScience & TechnologyLife Sciences & BiomedicineNutrition & DieteticsNF-?BNF-KAPPA-BOXIDATIVE STRESSNITRIC-OXIDETNF-ALPHAPOLYSACCHARIDESACTIVATIONCELLSCONSTITUENTSINFLAMMATIONEXPRESSION1001 Agricultural Biotechnology1111 Nutrition and DieteticsJournal Article10.3389/fnut.2021.7480312022-11-27Copyright: The authors34631774 (pubmed)http://purl.org/eprint/accessRights/OpenAccess2296-861X