Mathai, SamHarris, PaulBrimble, MargaretGunn, AlistairGuan, Jian2018-10-102014-12-09Journal of Experimental Stroke and Translational Medicine 6: 09 Dec 20141939-067Xhttp://hdl.handle.net/2292/40165Background and Purpose: Hypoxic -ischemic brain injury, due to reduced supply of oxygen, is a major cause of death and disability. There is no exclusive treatment available so far. Glycine-2-Methylproline-Glutamate(G-2MePE, NNZ 2566) , an analogue of Glycine-Proline-Glutamate reduces neuronal injury after focal ischemia in adult rats. The current study investigated into the neuroprotective effects of G-2MePE (1.2mg/kg) 3h post hypoxic-ischemic brain injury or the same volume of normal saline. Brains were extracted 5 days after the treatment. Tissue damage in the cortex, hippocampus and striatum was assessed. Neuronal survival, glial reactions, caspase-3 activity and TNF-a cytokine activity were also assessed. Results: The treatment with G-2Me-PE was associated with a significant reduction of tissue damage, improvement in neuronal survival, reduction in reactive microglia, TNF-a positive cells and caspase-3 positive cells in hippocampus and cortex but an elevation of astrocytosis. Conclusion: Neuroprotection with G-2MePE after hypoxic -ischemic brain injury in adult rats is associated with reduced neuronal necrosis, apoptosis, modulated inflammatory response and augmented astrocytosis.Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htmhttps://creativecommons.org/licenses/by-nc/3.0/Journal ArticleCopyright: Society for Experimental Strokehttp://purl.org/eprint/accessRights/OpenAccess