Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): Two complementary techniques for enzyme evolution

Bergquist, Peter; Reeves, RA; Gibbs, Moreland

Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): Two complementary techniques for enzyme evolution

Bergquist, Peter ; Reeves, RA ; Gibbs, Moreland

Identifier: http://hdl.handle.net/2292/7069

Issue Date: 2005

Reference: Biomol Eng 22(1-3):63-72 Jun 2005

Rights: Copyright: 2005 Elsevier B.V.

Rights (URI): https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm

Abstract:

Improvement of the biochemical characteristics of enzymes has been aided by misincorporation mutagenesis and DNA shuffling. Many gene shuffling techniques result predominantly in the regeneration of unshuffled (parental) molecules. We describe a proc

Show full item record