Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): Two complementary techniques for enzyme evolution

Bergquist, Peter; Reeves, RA; Gibbs, Moreland

dc.contributor.author	Bergquist, Peter	en
dc.contributor.author	Reeves, RA	en
dc.contributor.author	Gibbs, Moreland	en
dc.date.accessioned	2011-07-24T21:47:35Z	en
dc.date.issued	2005	en
dc.identifier.citation	Biomol Eng 22(1-3):63-72 Jun 2005	en
dc.identifier.issn	1389-0344	en
dc.identifier.uri	http://hdl.handle.net/2292/7069	en
dc.description.abstract	Improvement of the biochemical characteristics of enzymes has been aided by misincorporation mutagenesis and DNA shuffling. Many gene shuffling techniques result predominantly in the regeneration of unshuffled (parental) molecules. We describe a proc	en
dc.language	EN	en
dc.relation.ispartofseries	Biomolecular Engineering	en
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1389-0344/	en
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm	en
dc.subject	polymerase chain reaction	en
dc.subject	primer extension	en
dc.subject	in vitro evolution	en
dc.subject	gene shuffling	en
dc.subject	flow cytometry	en
dc.subject	random mutation	en
dc.subject	BACILLUS-CIRCULANS XYLANASE	en
dc.subject	DIRECTED EVOLUTION	en
dc.subject	BINDING DOMAINS	en
dc.subject	KRAFT PULP	en
dc.subject	IDENTIFICATION	en
dc.subject	EXPRESSION	en
dc.subject	BACTERIAL	en
dc.subject	SEQUENCE	en
dc.subject	PK(A)	en
dc.subject	CELLS	en
dc.title	Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): Two complementary techniques for enzyme evolution	en
dc.type	Journal Article	en
dc.identifier.doi	10.1016/j.bioeng.2004.10.002	en
pubs.issue	1-3	en
pubs.begin-page	63	en
pubs.volume	22	en
dc.description.version	AM - Accepted Manuscript	en
dc.rights.holder	Copyright: 2005 Elsevier B.V.	en
dc.identifier.pmid	15857785	en
pubs.end-page	72	en
dc.rights.accessrights	http://purl.org/eprint/accessRights/OpenAccess	en
pubs.subtype	Article	en
pubs.elements-id	8262	en
pubs.record-created-at-source-date	2010-09-01	en
pubs.dimensions-id	15857785	en